Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Srebf2

Cell type

Cell type Class
Neural
Cell type
Oligodendrocytes
NA
NA

Attributes by original data submitter

Sample

source_name
differentiated oligodendrocyte
cell type
differentiated oligodendrocyte from neural stem cell
passages
8
strain
FvB/C57BL6 hybrid
genotype
wild type
chip antibody
Srebp2 (10007663, Cayman Chemical)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, sonicated and then incubated with Qki-5, Srebp2, and Pol II antibodies. Protein-DNA complex were washed and ChIP'ed DNA were isolated for library preparation. Libraries were prepared by KAPA Hyper Prep Kit (KK8504, Kapa Biosystems).

Sequencing Platform

instrument_model
HiSeq X Ten

mm10

Number of total reads
22432648
Reads aligned (%)
80.7
Duplicates removed (%)
21.1
Number of peaks
228 (qval < 1E-05)

mm9

Number of total reads
22432648
Reads aligned (%)
80.7
Duplicates removed (%)
21.0
Number of peaks
193 (qval < 1E-05)

Base call quality data from DBCLS SRA